MdBioLab - Laboratory Activities - Mystery of the Crooked Cell

Mystery of the Crooked Cell

Specifications

  • Skill Level: Intermediate
  • Focus: Genetics, cell biology and physiology
  • Time: 50 – 70 minutes
  • Restrictions: None

Key Words

hemoglobin, gel, electrophoresis, symptoms, patient, agarose, sickle, family, disease, protein, genotype, phenotype, Punnett square, point mutation, homozygous, heterozygous, dominant, recessive

Summary

Dr. James Herrick of Chicago needs help diagnosing a patient. This patient, who feels well the majority of the time, has reported specific symptoms that lead Dr. Herrick to believe that he has sickle cell anemia. A look at his immediate family reveals that neither of his parents have the same symptoms. Upon further inspection, however, it is revealed that both the patient's uncle and grandmother have had the same symptoms under the same circumstances.

Dr. Herrick has asked for your classes help in confirming his diagnosis. To do so they will be testing the patient's hemoglobin protein using gel electrophoresis.

Objectives

Upon completion of The Mystery of the Crooked Cell students will be able to:

Pre-Lab

  • illustrate how amino acids interact to create a protein
  • explore the hemoglobin protein and its physiology

Laboratory

  • recognize the symptoms of sickle cell anemia and hemoglobin's role in the disease
  • differentiate between a patient with sickle cell anemia and a patient without sickle cell anemia using gel electrophoresis
  • calculate the probability of an offspring having a specific phenotype using a Punnett square
  • analyze a pedigree to track a gene through a family

Post-Laboratory

  • show how a point mutation can change the function of a protein

Additional Resources

Quickgrabs

Follow